Thionin Feulgen Stain for DNA (Ploidy) Quantitation by Image Analysis
作者:
WhitakerB. P.,
StiglianoW. W.,
CarsonF. L.,
LynnJ. A.,
期刊:
Journal of Histotechnology
(Taylor Available online 1993)
卷期:
Volume 16,
issue 2
页码: 113-116
ISSN:0147-8885
年代: 1993
DOI:10.1179/his.1993.16.2.113
出版商: Taylor&Francis
关键词: DNA;Feulgen;image analysis;ploidy
数据来源: Taylor
摘要:
AbstractWe describe the method and application of a reliable thionin Feulgen stain for paraffin sections of formalin fixed, routinely processed surgical pathology specimens. The technique substitutes thionin for basic fuchsin in the classical Schiff reagent and modifies the hydrolysis times. Serial sections cut from selected paraffin blocks were processed with both a commercial kit stain and the thionin Feulgen stain. Computer assisted image analysis was used to perform a direct comparison of tissue response to the two stains. The thionin Feulgen reagent yielded results as reliable as those from the commercial kit stain, at approximately one-fifth the cost. (The J Histotechnol16:113, 1993)
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