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An Enzyme Biosensor for Rapid Assay of Glucosinolates

 

作者: A. Koshy,   H.P. Bennetto,   G.M. Delaney,   A.J. Macleod,   J.R. Mason,   J.L. Stirling,   C.F. Thurston,  

 

期刊: Analytical Letters  （Taylor Available online 1988）
卷期: Volume 21, issue 12  

页码: 2177-2194

 

ISSN:0003-2719

 

年代: 1988

 

DOI:10.1080/00032718808059901

 

出版商: Taylor & Francis Group

 

关键词: amperometric;biosensor;enzyme electrode;glucose oxidase;glucosinolates;myrosinase;progoitrin;sinigrin

 

数据来源: Taylor

 

摘要:

A novel electrochemical method for analysis of glucosinolates is described. Glucose, released by the action of myrosinase on the analyte, is detected amperometrically by a glucose electrode having glucose oxidase immobilised on a platinised carbon base. The dependence of current responses on analyte concentration was linear up to 5mM for sinigrin and progoitrin, and the applicability of the method for determination of total glucosinolate in rape seed was demonstrated. An alternative approach was also examined which employed a bi-enzyme electrode made by co-immobilising the two enzymes on the same electrode.

 

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