A Novel Fluorometric Method for DNA and RNA Determination
作者:
W.-Y Li,
J.-G. Xu,
X.-Q. Guo,
Q.-Z. Zhu,
Y.-B. Zhao,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 3
页码: 527-536
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708001799
出版商: Taylor & Francis Group
关键词: DNA;RNA;Methylene Blue;Fluorescence Quenching
数据来源: Taylor
摘要:
A method for the determination of nucleic acids with methylene blue(MB) as a fluorescence probe was developed, based on the quenching of the fluorescence of MB in the presence of nucleic acids. The calibration graph was linear in the range of 0–40 μg/ml for CT DNA and 0–55 μg/ml for yeast RNA, the limit of detection was 28 ng/ml for CT DNA and 82 ng/ml for yeast RNA. CT DNA could be determined in the presence of 30%(w/w) yeast RNA and yeast RNA was determined when the content of CT DNA in synthetic samples was below 5%(w/w).
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