Characteristics of an Uptake System for α‐Aminoisobutyric Acid inLeishmania tropicaPromastigotes1
作者:
PEGGY R. LEPLEY,
ANTONY J. MUKKADA,
期刊:
The Journal of Protozoology
(WILEY Available online 1983)
卷期:
Volume 30,
issue 1
页码: 41-46
ISSN:0022-3921
年代: 1983
DOI:10.1111/j.1550-7408.1983.tb01030.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
ABSTRACT.Leishmania tropicapromastigotes transport α‐aminoisobutyric acid (AIB), the nonmetabolizable analog of neutral amino acids, against a substantial concentration gradient. AIB is not incorporated into cellular material but accumulates within the cells in an unaltered form. Intracellular AIB exchanges with external AIB. Various energy inhibitors (amytal, HOQNO, KCN, DNP, CCCP, and arsenate) and sulfhydryl reagents (NEM, pCMB, and iodoacetate) severely inhibit uptake. The uptake system is saturable with reference to AIB‐and the Lineweaver‐Burk plots show biphasic kinetics suggesting the involvement of two transport systems. AIB shares a common transport system with alanine, cysteine, glycine, methionine, serine, and proline. Uptake is regulated by feedback inhibition and transinhi
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