Simultaneous Determination of Glucose and Sucrose using a Flow System with Two Enzyme Reactors and an Octadecylsilica Column in One Line
作者:
Hisakazu Mori,
Mamie Kogure,
Yohko Kuroda,
Miyuki Nissato,
Yukiko Tamura,
Nahoko Watanabe,
Haruhiko Yamamoto,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 8
页码: 1531-1541
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542913
出版商: Taylor & Francis Group
关键词: Glucose;Sucrose;Simultaneous Determination;Enzyme Reactor;Octadecylsilica Column
数据来源: Taylor
摘要:
Simultaneous determination of glucose and sucrose was achieved with an apparatus containing two enzyme reactors and an octadecylsilica column positioned between the two reactors in tandem in a single flow line. The enzymes used were mutarotase and glucose dehydrogenase for glucose analysis, and sucrose phosphorylase, phosphoglucomutase and glucose-6-phosphate dehydrogenase for sucrose analysis. The pH and the concentration of the phosphate buffer used as the medium for immobilization of mutarotase and glucose dehydrogenase to activated aminopropyl glass were studied. Several aqueous buffers and their pH values were also examined for use as the carrier medium, and HEPES buffer (pH 7.5) was the most favorable. In this apparatus under these conditions, NADH due to glucose and that due to sucrose were observed separately. The calibration curve for glucose was linear in the range of 1 - 500 μM, and that for sucrose, 5 - 500 μM. This method was applied to the analyses of glucose and sucrose in several beverages. The results were in good agreement with those obtained by a commercially available test-kit method.
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