A Method for Histological Preparation of Undecalcified Bone Sections Containing Acrylic Bone Cement
作者:
JensenLars Nimb,
JensenJøRgen Steen,
GotfredsenKlaus,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 2
页码: 82-86
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110555
出版商: Taylor&Francis
关键词: bone cement;biocompatibility and polymethylmethacrylate (PMMA);histological evaluation
数据来源: Taylor
摘要:
An improved and time reducing method is presented for the histological evaluation of bone containing polymethylmethacrylate (PMMA) bone cement. The undecalcified bone was embedded in epoxy resin and sections of 50-100μm thickness were produced using a commercially available cutting grinding system. The sections were stained with Stevenel's blue and van Gieson picrofuchsin or a modified hematoxylin-eosin. PMMA bone cement was not dissolved and remained enabling examination in situ of an intact cement bone interface and tissue reaction without decalcification.
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