To pursue the possibility that subdivisions within the medial and lateral septal nuclei are differentially involved in adrenocortical function, plasma samples obtained prior to and following electrical stimulation of the septal nuclei of urethane (1.30 g/kg)-anesthetized female rats were assessed for corticosterone concentration. Hippocampal EEG, ECG, heart rate (HR), mean arterial pressure (MAP) and respiration were routinely monitored, and timed blood samples (0.15 ml) were obtained from a catheterized tail artery. Blood samples were taken 0.5 min prior to and at 5, 10, 15 and 30 min after initiation of stimulation. Whereas no change in Cpd B levels were observed following sham stimulation or stimulation of the corpus callosum, fornix or anterior commissure, stimulation of the septal nuclei produced differential responses. Decreased plasma Cpd B responses followed stimulation of the medial septal nucleus (MS); increases in plasma Cpd B followed stimulation of the dorsal (LSD) and ventral (LSV) division of the lateral septal nucleus. The overall increase in plasma Cpd B levels following LSD and LSV stimulation was 16 and 32%, respectively. The overall decrease in corticosterone concentration subsequent to MS stimulation was 18%. The largest increases in Cpd B levels occurred at 5 min (36%) and 10 min (24%) for LSV and LSD groups, respectively; the largest decrease was noted at 15 min (25%). Plasma Cpd B responses to stimulation of the intermediate area of the lateral septal nucleus produced varying and inconsistent responses. Collectively, these data support the hypothesis that subdivisions within the septal nuclei are differentially involved in adrenocortical function.