A hydrochemical and environmental isotopes study was undertaken for Mallawi area in El-Minia province in upper Egypt. The results of hydrochemical analysis indicate that the salinity ranges from 200–950 ppm and that the predominate water type is meteoric and mixed water. The interrelation between different anions and cations were discussed in terms of probable different contribution of dissolution of either halide minerals or gypsum or limestone rocks. The correlation between the deuterium content and the salinity as well as the major anions has been studied to determine the recharge source. The results indicate a trend of decrease of salinity chloride and sulphate content with increasing deuterium concentration, while the bicarbonate shows the opposite relationship. The hydrochemical and environmental isotopes data point out a recent recharge of groundwater with some contribution of palaeowater (characterized by low content of deuterium, oxygen 18 and no tritium). This contribution is different from well to another and is clearer in the Eastern fringe than in the Western Part of the River Nile Valley.