Xylem-limited bacteria (XLB) were recovered from the sharpshooter leafhoppers,Homalodisca coagulata(Say),Oncometopia orbona(F.), andParaulacizes irrorata(F.), and cultured on artificial agar medium. Seasonal occurrence of XLB inH. coagulataand its plant hosts were determined by in vitro isolations and enzyme-linked immunosorbent assays (ELISA) for XLB. XLB were recovered frequently fromH. coagulatafrom numerous feeding hosts soon after they moved into peach orchards. Highest recoveries of XLB from host plants were from commercial plums,Prunus salicinaL. (70.1%), that showed visible leaf scald symptoms, followed by peaches,Prunus persica(L. [Batsch[) (31.4%).