The karyotypes of the three main domestic Bovidae species, Bos taurus, Ovis aries, and Capra hircus, have been investigated by RBG-banding. Primary fíbroblast cells were cultured from fetal lung or muscle tissue and collected for chromosome preparation after double thymidine synchronization. Depending on the culture and species, BrdU and FdU were added for 8–11 h or 7–10 h, respectively, before harvest. The chromosomes of the three species were classified and numbered according to standard conventions (ISCNDA, 1989) and compared at different stages of condensation. Within the resolving power of the images obtained, the RBG-banding patterns appear to be very similar, with only minor differences existing between chromosome 9 and the sex chromosomes of cattle and the same chromosomes in the domestic sheep and the