Cytotoxicity and mutagenicity of dimethylnitrosamine in mammalian cells (CHO/HGPRT system): Enhancement by calcium phosphate
作者:
J. Patrick O'Neill,
Richard Machanoff,
Juan R. San Sebastian,
Abraham W. Hsie,
期刊:
Environmental Mutagenesis
(WILEY Available online 1982)
卷期:
Volume 4,
issue 1
页码: 7-18
ISSN:0192-2521
年代: 1982
DOI:10.1002/em.2860040103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: CHO/HGPRT system;mutagenicity;dimethylnitrosamine;CHO cells;mammalian cells;thioguanine resistance;S9 metabolism
数据来源: WILEY
摘要:
AbstractThe cytotoxicity and mutagenicity of dimethylnitrosamine (DMN) was determined in the CHO/HGPRT system. Metabolic activation of the promutagen was achieved by use of a liver homogenate supernatant (S9) prepared from Aroclor 1254‐induced Sprague‐Dawley rats. The cytotoxic and mutagenic effects of DMN were enhanced by the inclusion of calcium chloride in the incubation mix, and this enhancement was dependent on the presence of sodium phosphate. Under conditions that yielded maximal activity (10 mM calcium chloride, 10 mM magnesium chloride, 50 mM sodium phosphate), an apparent calcium phospate precipitate was observed. DMN activity increased with increasing amounts of S9 protein over the range 0.3–3.0 mg/ml in the S9 mix and appeared to plateau at higher concentrations. The mutagenicity of DMN can be described as 110 mutants/106cells per mM DMN per mg/ml S9 protein per
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