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Possibilities and Scope of the Double Isotope Effect Method in the Elucidation of Mechanisms of Enzyme Catalyzed Reactions

 

作者: H.-L. Schmidt,   R. Medina,  

 

期刊: Isotopenpraxis Isotopes in Environmental and Health Studies  （Taylor Available online 1991）
卷期: Volume 27, issue 1  

页码: 1-4

 

ISSN:0021-1915

 

年代: 1991

 

DOI:10.1080/10256019108622450

 

出版商: Taylor & Francis Group

 

关键词: arginase;biochemical reaction kinetics;biochemistry;carbon 13;dehydrogenases;enzymes;isotope effects;isotope ratio;lactate dehydrogenase;metabolism;nitrogen 15;pyruvic acid;oxygen 18;stable isotopes;urease

 

数据来源: Taylor

 

摘要:

Kinetic isotope effects on enzyme catalyzed reactions are indicative for the first irreversible in a sequence of individual steps. Hints on the relative velocities of other steps can only be obtained from the partitioning factor R and its dependence on external reaction conditions. In general, the experimental data needed are obtained from isotope abundance measurements in a defined position of the substrate or product as a function of turnover. This method does not reveal events dealing with neighbour atoms or preceeding the main isotope sensitive step.

 

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