首页   按字顺浏览 期刊浏览 卷期浏览 Progress in sequencing deoxyribonucleic acid with an atomic force microscope
Progress in sequencing deoxyribonucleic acid with an atomic force microscope

 

作者: H. G. Hansma,   A. L. Weisenhorn,   S. A. C. Gould,   R. L. Sinsheimer,   H. E. Gaub,   G. D. Stucky,   C. M. Zaremba,   P. K. Hansma,  

 

期刊: Journal of Vacuum Science&Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena  （AIP Available online 1991）
卷期: Volume 9, issue 2  

页码: 1282-1284

 

ISSN:1071-1023

 

年代: 1991

 

DOI:10.1116/1.585221

 

出版商: American Vacuum Society

 

关键词: BIOPHYSICS;ATOMIC FORCE MICROSCOPY;DNA SEQUENCING;USES;NUCLEOTIDES;RESOLUTION;SAMPLE PREPARATION

 

数据来源: AIP

 

摘要:

Atomic force microscope (AFM) images of single‐stranded deoxyribonucleic acid (DNA) showing nucleotide resolution have been obtained using two sample preparation methods: DNA covalently attached to a polymerized lipid monolayer and then imaged under water and DNA on mica rinsed with a Ba(NO3)2solution and then imaged under ethanol. Some of the bases were identified in the AFM image of DNA on polymerized lipid. Current problems in sequencing DNA with the AFM include movement of the DNA during imaging and the difficulty of reproducing experiments.

 

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