Differential Endothelial Migration and Proliferation to Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor
作者:
YoshidaAtsushi,
AnandBela,
ZetterBruce R.,
期刊:
Growth Factors
(Taylor Available online 1996)
卷期:
Volume 13,
issue 1-2
页码: 57-64
ISSN:0897-7194
年代: 1996
DOI:10.3109/08977199609034566
出版商: Taylor&Francis
关键词: angiogenesis;endothelium;chemotaxis;chemokinesis;proliferation
数据来源: Taylor
摘要:
AbstractNeovascularization is a feature of a variety of pathological processes. We compared the characteristics of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on migration and proliferation of human umbilical vein endothelium (HUVEC). Both VEGF and bFGF induced endothelial cell migration at similar concentrations (½max. VEGF =∼1.0 ng/ml, bFGF =∼5.0 ng/ml). However, VEGF-stimulated migration was two-fold greater than bFGF at 1 and 10 ng/ml (p<0.05). In contrast, bFGF induced proliferation four-fold more effectively than VEGF (½max. 1 ng/ml and 1.4 ng/ ml respectively). Checkerboard migration assays for bFGF showed a predominantly chemokinetic pattern, whereas VEGF was predominantly chemotactic. VEGF and bFGF were not synergistic in monolayer proliferation and migration assays. Three angiogenesis inhibitors, alpha-interferon, TNP-470, and platelet factor-4, inhibited VEGF and bFGF induced cell migration. These results indicate that VEGF and bFGF are chemoattractants that stimulate endothelial migration by different mechanisms and that both can be inhibited by known angiogenesis inhibitors.
点击下载:
PDF (728KB)
返 回