The histochemistry of mucins in the submaxillary salivary gland has been studied in 10 buffaloes of different age groups. The acidic mucins exhibited moderate to intense reaction in new-born and young animals; in adult animals, these were noticed in patches. Neutral and non-sulphated mucins were observed in traces and in mild amounts, respectively, in the mucous secretory end-pieces of different age groups; sialomucins were seen in moderate to intense concentrations. The seromucous end-pieces revealed traces of neutral mucins in new-born buffalo calves and moderate to intense concentrations in young and adult animals. These structures showed mild concentration of acidic mucins, hyaluronic acid and sialomucins. Sulphated and non-sulphated mucins were observed in traces in the seromucous end-pieces. The apical border of the lining cells of the intra- and interlobular ducts contained moderate to intense concentrations of acidic mucins. In young and adult animals, the epithelial cells showed mild to moderate reaction for neutral mucins. Goblet cells of the interlobular ducts exhibited the presence of mild and intense concentrations of sialo- and sulphomucins, respectively.