EMBRYOGENIC DETERMINATION AND RIBONUCLEIC ACID SYNTHESIS IN POLLEN GRAINS OF HYOSCYAMUS NIGER (HENBANE)
作者:
V. Raghavan,
期刊:
American Journal of Botany
(WILEY Available online 1979)
卷期:
Volume 66,
issue 1
页码: 36-39
ISSN:0002-9122
年代: 1979
DOI:10.1002/j.1537-2197.1979.tb06190.x
出版商: Wiley
数据来源: WILEY
摘要:
3H‐uridine administered as a one‐ or two‐hour pulse to embryogenic pollen grains of freshly excised anthers ofHyoscyamus niger(henbane) was autoradiographically localized in embryoids formed during a subsequent chase. Although continuous incubation of anthers in actinomycin D inhibited embryogenesis, a small percentage of potentially embryogenic pollen escaped inhibition if anthers were grown for at least one hour in the basal medium before actinomycin treatment. The results imply that certain pollen grains become embryogenically determined immediately after culture of the anther and that this is accompanied by the synthesis of ribonucleic acid.
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