Concentration of baboon endogenous virus in large‐scale production by use of hollow‐fiber ultrafiltration technology
作者:
S. A. Weiss,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1980)
卷期:
Volume 22,
issue 1
页码: 19-31
ISSN:0006-3592
年代: 1980
DOI:10.1002/bit.260220103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow‐fiber ultrafiltration technology under conditions of large‐scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5‐fold during concentration. Thermal inactivation of BaEV was retarded by the addition of lactose glutamate to the harvested tissue culture fluid. After concentration, at least 91% of the virus RNA‐directed DNA polymerase is recovered with a concomitant increase in infectious virus. Materials needed for modifying described systems may be obtained from commercial
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