The gene encoding the 133 kDa insecticidal crystal protein ofBacillus thuringiensisvar.kurstakiisolate DSIR732, has been cloned intoEscherichia coli. The complete nucleotide sequence of the coding region of thecry732 gene, has been determined and indicated a large open reading frame (ORF) of 3534 nucleotides encoding a protein of 1178 amino acid residues, with an estimated molecular weight of 133 330 Da. In addition, the sequence of 387 nucleotides 5′ and 257 nucleotides 3′ of the coding region has been determined. Sequence comparisons of thiscry732 gene and its flanking regions with those of other Btcrygenes revealed that it is a member of thecryIA(c) class of Btcrygenes. Alignment of thecry732 nucleotide sequence with that of thecryIA(c) holotype gene sequence from BtkurstakiHD73 revealed only four nucleotide differences in the ORF, none of which resulted in amino acid substitutions, and one nucleotide difference in the region 5′ to the ORF. Nucleotide sequence comparisons suggested that thecry732 gene may have resulted from recombination events between thecryIA(c) genes of BtkurstakiHD73 and BtkenyaeHD558—2. Thecry732 gene has been expresed inE. coliand bioassays, using extraas ofE. coliproducing the 133 kDa Cry732 protein, have demonstrated that the recombinant protein retains its insecticidal properties against three species of leafrollers (Lepidoptera: Tortricidae).