High‐throughput purity estimation and characterisation of synthetic peptides by electrospray mass spectrometry
作者:
SWEE S. SMART,
TOM J. MASON,
PAUL S. BENNELL,
N. JOE MAEIJ,
H. MARIO GEYSEN,
期刊:
International Journal of Peptide and Protein Research
(WILEY Available online 1996)
卷期:
Volume 47,
issue 1‐2
页码: 47-55
ISSN:0367-8377
年代: 1996
DOI:10.1111/j.1399-3011.1996.tb00809.x
出版商: Blackwell Publishing Ltd
关键词: electrospray mass spectrometry;high‐throughput analysis;multiple synthesis;peptide analysis
数据来源: WILEY
摘要:
High‐throughput analysis for purity and molecular weight determination of synthetic peptides including characterisation of any peptidic byproducts arising from synthesis is described. The data from electrospray mass spectrometry are processed with an algorithm that calculates the contribution of the target peptide and each of the identifiable contaminants to the total ionizable material in a sample of synthetic peptide. All essential data are obtained by one instrumental technique in<3 min per sample. The technique has distinct advantages in the rapid analysis of the many hundreds of peptides/peptidomimetics required in systematic quantitative structure‐activity relationship and other investigations. ©Munksgaard
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