Determination of Clozapine and Its Two Major Metabolites in Human Serum by Liquid Chromatography Using Ultraviolet Detection
作者:
Uma Hariharan,
M. Hariharan,
JanakiS. Naickar,
Rajiv Tandon,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 15
页码: 2409-2417
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608014026
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new, simple, reverse phase, and highly reproducible HPLC-UV method that has all the comprehensive features of good column, mobile phase, internal standard and extraction has been developed for the assay of clozapine (CLZ), norclozapine (NCLZ) and clozapine-N-oxide (CLZNO) in human serum. The method is very easy to adapt, overcomes the problems of earlier methods and is very economical. Amoxapine is the internal standard. All three analytes are extracted from alkaline serum using ethyl acetate and the absolute extraction efficiency is ∼95%. The UV detector is set at 230 nm. The mobile phase is a mixture of phosphate buffer (0.05M, pH 2.7), acetonitrile and methanol (62:20:18 v/v) and contains 2.5 mL triethylamine per liter of solution. The detection limit of the method is 2 ng/mL for CLZ and NCLZ and 4 ng/mL for CLZNO. The mean CV for the method is 5%.
点击下载:
PDF (342KB)
返 回