首页   按字顺浏览 期刊浏览 卷期浏览 Real‐time area‐tracker records cellular volume changes from video images
Real‐time area‐tracker records cellular volume changes from video images

 

作者: Bernd Lindemann,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1984)
卷期: Volume 55, issue 11  

页码: 1788-1790

 

ISSN:0034-6748

 

年代: 1984

 

DOI:10.1063/1.1137660

 

出版商: AIP

 

数据来源: AIP

 

摘要:

High‐contrast TV images of living cells are recorded from a light microscope. The video line signal is converted to binary and used to gate a 12.5‐MHz clock driving a counter. After completion of each video frame the accumulated counts (proportional to the dark image area) are written onto a stack (FIFO) before the counter is reset. Thus, area information is actualized at a rate of 50 values per second and can be read out at speeds suitable for computer interfacing and (after D/A conversion) oscilloscope displays or paper chart recording. With two counting channels the time course of two areas of distinctly different gray shades can be recorded at once.

 

点击下载:  PDF (232KB)



返 回