首页
按字顺浏览
期刊浏览
卷期浏览
Aerobic TNT Reduction via 2-Hydroxylamino-4,6-Dinitrotoluene byPseudomonas aeruginosaSt...
|
Aerobic TNT Reduction via 2-Hydroxylamino-4,6-Dinitrotoluene byPseudomonas aeruginosaStrain MX Isolated from Munitions-Contaminated Soil
作者:
Galina K. Vasilyeva,
Byung-Taek Oh,
Patrick J. Shea,
Rhae A. Drijber,
Vladimir D. Kreslavski,
Robert Minard,
Jean-Marc Bollag,
期刊:
Bioremediation Journal
(Taylor Available online 2000)
卷期:
Volume 4,
issue 2
页码: 111-124
ISSN:1088-9868
年代: 2000
DOI:10.1080/10889860091114176
出版商: TAYLOR & FRANCIS
数据来源: Taylor
摘要:
Bioremediation of munitions-contaminated soil requires effective transformation and detoxification of high concentrations of 2,4,6-trinitrotoluene (TNT).Pseudomonas aeruginosastrain MX, isolated from munitions-contaminated soil, aerobically transformed TNT (100 mg/L) in culture medium within 15 h, causing transient accumulation of hydroxylaminodinitrotoluenes (HADNTs). The predominance of 2-hydroxylamino-4,6-dinitrotoluene (2HADNT), as well as 2-amino-4,6-dinitrotoluene (2ADNT) and 4,4′,6,6′ -tetranitro-2,2′ -azoxytoluene (2,2′AZT), indicated preferential reduction of the TNTorthonitro group. While only 12% of the TNT was transformed to 2ADNT, up to 65% was transformed to tetranitroazoxytoluenes (AZTs), which accumulated as a precipitate. The precipitate was formed by microscopic particles adhering to bacterial cells, which subsequently formed clusters containing lysed cells. Toxicity toward bacteria was primarily attributed to 2ADNT, because pure AZTs preincubated with sterile medium had little effect on the strain. While the culture medium containing TNT exhibited toxicity toward corn (Zea maysL.) and witchgrass (Panicum capillareL.), little phytotoxicity was observed after incubating withP. aeruginosastrain MX for 4 d. Strong binding of HADNTs to soil and low AZT bioavailability may further promote the detoxification of TNT in soil.
点击下载:
PDF (133KB)
返 回
|
|