Human Sperm Surface Mapping with Lectins
作者:
U.K. Bains,
S. Sehgal,
S.R. Bawa,
期刊:
Cells Tissues Organs
(Karger Available online 1992)
卷期:
Volume 145,
issue 3
页码: 207-211
ISSN:1422-6405
年代: 1992
DOI:10.1159/000147367
出版商: S. Karger AG
关键词: Human spermatozoa;Lectins;Fluorescence/electron microscopy;Flow cytometry
数据来源: Karger
摘要:
Ten fluorescein isothiocyanate (FΙTC)-linked lectins [Bauhimia purpurea, Concanavalin A, Dolichos biflorus (DBA), Griffonia simplicifolia I, Griffonia simplicifolia II, Madura pomifera, Arachis hypogea (PNA), Glycine max, Ulex europaeus (UEA) and Triticum vulgaris agglutinin] have been used to study their binding features on the human ejaculate spermatozoa. Qualitative changes in the labeling pattern have been observed in unfixed and acetone-treated spermatozoa. Furthermore, ultrastructural localization of some of the colloidal gold-linked lectins, namely PNA, UEA and DBA, has been attempted to delineate the binding domains of the specific sugars on the sperm surface. It needs to be emphasized that flow-cytometric methods employed in our study, which provide quantitative slant to qualitative data, should be utilized to evaluate the functional status of the spermatozoa
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