A novel structured kinetic modeling approach for the analysis of plasmid instability in recombinant bacterial cultures
作者:
William E. Bentley,
Dhinakar S. Kompala,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1989)
卷期:
Volume 33,
issue 1
页码: 49-61
ISSN:0006-3592
年代: 1989
DOI:10.1002/bit.260330108
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe instantaneous specific growth rate of a recombinant bacterial culture is directly calculated using a simple structured kinetic modeling approach. Foreign plasmid replication and foreign protein expression represent metabolic burdens to the host cell. The individual effects of these plasmid‐mediated activities on the growth rate of plasmid‐bearing cells are estimated separately. The dynamic and steady state simulations of the model equations show remarkable agreement with widely observed experimental trends in plasmid copy number and foreign protein content. The model provides an important tool for understanding and controlling plasmid instability in recombinant bacterial fermentations. The modeling framework employed here is suitable for studying the metabolism and growth of a variety of microbial cultu
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