Anemia Induces 5’-Nucleotidase in Fibroblasts of Cortical Labyrinth of Rat Kidney
作者:
M. Le Hir,
K.-U. Eckardt,
B. Kaissling,
期刊:
Kidney and Blood Pressure Research
(Karger Available online 1989)
卷期:
Volume 12,
issue 5-6
页码: 313-319
ISSN:1420-4096
年代: 1989
DOI:10.1159/000173208
出版商: S. Karger AG
关键词: Anemia;5’-Nucleotidase;Rat;Kidney;Adenosine;Immunohistochemistry;Hypoxia;Erythropoietin
数据来源: Karger
摘要:
We recently observed a strong increase of 5’-nucleotidase in renal fibroblasts of rats that were anemic due to an immunity against erythropoietin. In order to test if the change of 5’-nucleotidase was related with anemia, we studied the distribution of the enzyme in irradiated rats treated with phenylhydrazine. The hematocrit of these rats decrased to 15% within 4 days and erythropoietin levels were more than 200 times over controls. After 7 days a histochemical study showed that the enzymatic activity and the immunoreactivity for 5’-nucleotidase was markedly enhanced in the fibroblasts of the cortical labyrinth. There was no modification of 5’-nucleotidase in other cell types of the kidney. The 5’-nucleotidase activity of renal fibroblasts in cell culture increased by 72% upon addition of 160 µM 5’-AMP to the culture medium for 8 days. We propose that anemia provokes an energy deficit in some structure in the cortical labyrinth. This might increase the concentration of 5’-AMP which would induce 5’-nucleotidase. An interesting consequence of these events would be an increased production of adenosine in the direct vicinity of some of its putative targets, the glomerular arterioles and the erythropoietin
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