The Use of Fluorescent Nuclear Dyes for the Study of Blood Vessel Structure and Function: Novel Applications of Existing Techniques
作者:
Craig J. Daly,
John F. Gordon,
John C. McGrath,
期刊:
Journal of Vascular Research
(Karger Available online 1992)
卷期:
Volume 29,
issue 1
页码: 41-48
ISSN:1018-1172
年代: 1992
DOI:10.1159/000158930
出版商: S. Karger AG
关键词: Fluorescence;Hoechst 33342;Ethidium;Nuclei;Image analysis;Blood vessels
数据来源: Karger
摘要:
We have used nuclear fluorescent dyes to develop a technique for the study of vascular structure and function. Nuclear stained blood vessels, viewed with the appropriate filter sets, can be studied in great detail. Only the nuclei of the cells which form the walls are visible and so their positions relative to one another as well as their viability can be quickly assessed. The dyes are not toxic, therefore when the vessel contracts or relaxes, the changes in position of the nuclei can be monitored. In this paper we describe two original applications of fluorescent nuclear dyes in vascular research.
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