Formation of a multilayer cellular lining on a polyurethane vascular graft following endothelial cell sodding
作者:
Stuart K. Williams,
Thomas Carter,
Pauline K. Park,
Deborah G. Rose,
Timothy Schneider,
Bruce E. Jarrell,
期刊:
Journal of Biomedical Materials Research
(WILEY Available online 1992)
卷期:
Volume 26,
issue 1
页码: 103-117
ISSN:0021-9304
年代: 1992
DOI:10.1002/jbm.820260110
出版商: John Wiley&Sons, Inc.
数据来源: WILEY
摘要:
AbstractSmall‐diameter (<6 mm) clinically available vascular grafts often fail due in part to the inherent thrombogenicity of artificial polymers. Transplantation of endothelial cells onto the lumen of these vascular grafts has been suggested as one method to overcome this thrombogenicity. We have developed a compliant polyurethaneurea (PEUU) 4‐mm graft with a luminal surface modified by a glow discharge gas plasma. Autologous microvessel endothelial cells were isolated from canine falciform ligament fat, were transplanted onto the luminal surface of the grafts using an intraoperative isolation and sodding technique, and both endothelial‐cell‐treated and non‐cell‐treated grafts were placed as bilateral carotid interposition grafts in a canine model. After 5 weeks of implantation, explanted control (non‐cell‐treated) grafts exhibited a deposition of platelets, white cells and fibrin characteristic of a thrombogenic surface. MVEC sodded grafts exhibited a multicellular lining within but distinct from the lumen of the PEUU graft. The blood‐contacting surface of this lining exhibited an antithrombogenic endothelial cell monolayer. We suggest that the PEUU graft supported the initial deposition of MVEC and development of an endothelial cell lining. During the 5 weeks of implantation this lining continued to proliferate and detached from the PEUU graft substratum. The final neocellular lining exhibited a luminal diameter and histological features similar t
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