Production of wheat doubled haploids via wide crosses in New Zealand wheat
作者:
A. W. Campbell,
W. B. Griffin,
D. J. Burritt,
A. J. Conner,
期刊:
New Zealand Journal of Crop and Horticultural Science
(Taylor Available online 2000)
卷期:
Volume 28,
issue 3
页码: 185-194
ISSN:0114-0671
年代: 2000
DOI:10.1080/01140671.2000.9514138
出版商: Taylor & Francis Group
关键词: wheat;Triticum aestivum;maize;Zea mays;wide crosses;doubled haploids;breeding;genetics
数据来源: Taylor
摘要:
Doubled haploid (DH) technology allows the production of homozygous cereal lines in a single generation. The integration of DH technology into New Zealand wheat(Triticum aestivum)breeding and genetics programmes has the potential to reduce the breeding time of new cultivars and improve our understanding of agronomic ally important genetic traits. Wheat × maize(Zea mays)crosses are now widely used in the production of wheat DHs. The aim of this study was to develop a wheat × maize method of DH production using New Zealand germplasm. A number of New Zealand wheat cultivars were crossed with different maize genotypes. A successful method for producing wheat DHs was achieved with all genotypes. Several factors were identified as being important in the efficiency of wheat DH production. These included: seasonal constraints, time of embryo excision, method of auxin application, and culture media composition. Of these factors, seasonal constraints were the major limitation in the use of the wheat × maize method for producing DHs in New Zealand.
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