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A Rapid Method for Macerating Phloem

 

作者: PetersonCarol A.,   BlaisMichelle A.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1991）
卷期: Volume 66, issue 5  

页码: 242-245

 

ISSN:1052-0295

 

年代: 1991

 

DOI:10.3109/10520299109109980

 

出版商: Taylor&Francis

 

关键词: maceration;phloem;sieve elements;sieve cells;sieve tube members;sieve tubes;callose;aniline blue;fluorescence microscopy

 

数据来源: Taylor

 

摘要:

Sieve cells and sieve tube members can be macerated from the phloem of various organs of woody and herbaceous species by au-toclaving the tissue in a mild macerating medium. This treatment does not digest the primary walls or the callose deposits on the sieve areas and sieve plates of the sieve elements. These cells can then be recognized by the fluorescence of their callose after staining with aniline blue. Sometimes adjacent sieve elements fail to separate and one can observe details of their junctures.

 

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