Methylene Blue Prevents Pulmonary Injury after Intestinal Ischemia-Reperfusion
作者:
Yair Galili,
Ron Ben-Abraham,
Abraham Weinbroum,
Silvia Marmur,
Adrian Iaina,
Yoram Volman,
Gari Peer,
Oded Szold,
Dror Soffer,
Josef Klausner,
Micha Rabau,
Yoram Kluger,
期刊:
The Journal of Trauma: Injury, Infection, and Critical Care
(OVID Available online 1998)
卷期:
Volume 45,
issue 2
页码: 222-226
ISSN:0022-5282
年代: 1998
出版商: OVID
数据来源: OVID
摘要:
ObjectiveTo investigate the effect of methylene blue, an inhibitor of oxygen radicals, on lung injury caused by reperfusion of ischemic tissue.MethodsIntestinal ischemia-reperfusion injury was induced in rats by clamping the superior mesenteric artery for 1 hour. Thereafter, the experimental group was administered 1% methylene blue intraperitoneally and the control group received saline. After 4 hours, pulmonary histopathologic features were assessed, and lung wet-weight to dry-weight ratios and tissue xanthine oxidase were determined.ResultsThe control group suffered from severe pulmonary parenchymal damage, compared with slight damage in the experimental group. The number of sequestered neutrophils was significantly higher in the control group (319 +/- 60 polymorphonuclear cells per 10 high-power fields) than in the methylene blue-treated group (91 +/- 8 polymorphonuclear cells per 10 high-power fields; p < 0.001). The wet-weight to dry-weight ratio was significantly increased in the saline-treated rats compared with the methylene blue-treated group (6.19 +/- 0.28 vs. 5.07 +/- 0.21; p < 0.001). Xanthine oxidase activity was similar in both groups.ConclusionMethylene blue attenuated lung injury after intestinal ischemia-reperfusion. Inhibition of oxygen free radicals may be the protective mechanism.
返 回