Thiol adducts of ethacrynic acid increase outflow facility in enucleated calf eyes
作者:
EpsteinDavid L.,
HooshmandLeila Beth,
EpsteinMichael P.M.,
期刊:
Current Eye Research
(Taylor Available online 1992)
卷期:
Volume 11,
issue 3
页码: 253-258
ISSN:0271-3683
年代: 1992
DOI:10.3109/02713689209001776
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Ethacrynic acid (ECA), a sulfhydryl (SH)-reac-tive diuretic drug, has been shown to increase outflow facility (C) both in living monkey eyes and in the calf eye, in vitro (Epstein et al. 1987). In an attempt to increase the therapeutic index of this drug for potential clinical use in glaucoma, we explored the effect of various thiol adducts of ECA on C in the calf eye in vitro. These adducts might be expected to liberate ECA by a reversible retro-Michael type reaction.Enucleated calf eyes were perfused at 25°C at 15 mm Hg for 5 hours with various ECA-thiol adducts. ECA-cysteine at 0.25 mM (for each) increased outflow facility 104% compared to 38% in sham manipulated eyes (n-10; p<.005). A dose response effect was demonstrated from 0.01 mM to 0.25 mM. A relative potency table (for increasing C) was established for several ECA-thiol adducts:Cysteine = cysteamine
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