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An Enzymatic Chemiluminescence Optrode for Choline Detection Under Flow Injection Conditions

 

作者: H. Lapp,   U. Spohn,   D. Janasek,  

 

期刊: Analytical Letters  （Taylor Available online 1996）
卷期: Volume 29, issue 1  

页码: 1-17

 

ISSN:0003-2719

 

年代: 1996

 

DOI:10.1080/00032719608000387

 

出版商: Taylor & Francis Group

 

关键词: Chemiluminescence optrode;choline;bienzyme membrane;cholin;flow cell

 

数据来源: Taylor

 

摘要:

A sensitive choline sensor was developed on the basis of a chemiluminometric bienzyme optrode. Choline oxidase (ChOx) and fungal peroxidase (FPOD) were covalently immobilized on a preactivated nylon membrane. The addition of polyethylenimine to the mixed enzyme solution before the immobilization combined with the crosslinking by gaseous glutardialdehyde improves both the long-term stability and the sensitivity. Choline can be detected in the range between 10−6and 10−3M.

 

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