Interrelation between Oxygen Consumption and Na-K-ATPase Activity in Rat Renal Proximal Tubule Suspension
作者:
Gerhard Gstraunthaler,
Walter Pfaller,
Peter Kotanko,
期刊:
Kidney and Blood Pressure Research
(Karger Available online 1985)
卷期:
Volume 8,
issue 1
页码: 38-44
ISSN:1420-4096
年代: 1985
DOI:10.1159/000173032
出版商: S. Karger AG
关键词: Na-K-ATPase/O2;Oxygen consumption;Rat proximal tubule;Renal cortical tubular suspension
数据来源: Karger
摘要:
The portion of Na-K-ATPase activity on oxygen consumption was determined in suspensions of rat proximal tubules by monitoring oxygen consumption (QO2) under different metabolic states: in the presence or absence of succinate or lactate (10 mmol/l) and increasing concentrations of ouabain (0.4; 0.8; 1.2 and 1.6 mmol/l). In the metabolic states tested, the ouabain induced decrease of QO2 was identical, which implies a fixed rate between Na-K-ATPase activity and QO2. On the basis of these results, Ki and maximal inhibition rate were determined by Lineweaver-Burk and Eadie-Hofstee plots. Ki was 0.67 mmol/l ouabain and the maximal inhibition of QO2 was 77%. This corresponds to an absolute decrease of QO2 of 1,630 µmol O2 h-1 g-1 protein. Since this value represents the real portion of Na-K-ATPase on QO2, the activity of Na-K-ATPase can be calculated, yielding an activity of 163 µmol ATP min-1 g-1 tubule protei
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