Investigation of Peroxynitrite Induced Oxidative Stress in Red Blood Cells Monitored by Luminol-Dependent Chemiluminescence.
作者:
Jaroslaw Kanski,
Tanuja Koppal,
D. Allan Butterfield,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 6
页码: 1183-1192
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542887
出版商: Taylor & Francis Group
关键词: Chemiluminescence;Luminol;Peroxynitrite;Glutathione (GSH);Oxidative Stress (ROS);Red Blood Cells
数据来源: Taylor
摘要:
Peroxynitrite-induced oxidative stress was investigated in red blood cells by luminol-dependent chemiluminescence. Red blood cells are susceptible to free radical damage due to the abundance of oxygen, presence of iron and other agents such as polyunsaturated fatty acids. Peroxynitrite (ONOO-), formed by the reaction of superoxide radical with nitric oxide radical, has been shown to cause protein and lipid oxidation. Addition of ONOO- showed a dramatic increase in chemiluminescence, but treatment with glutathione ethyl ester, an antioxidant, partially reduced the increase in chemiluminescence. The results show that red blood cells are a good model to mimic oxidative stress conditions and are discussed with relevance to neurodegenerate disorders.
点击下载:
PDF (338KB)
返 回