The antimicrobial activities of sevenEpicoccum purpurascensstrains isolated either from evergreen oak leaves (Quercus ilex) collected over a period of one year, or from the atmosphere were comparedin vitro. Two strains sporulated and conspicuously inhibited the growth ofStaphylococcus aureusandTrichophyton mentagrophytes. Thin-layer chromatographic studies showed the existence of some compounds, such as flavipin, which were common to all the strains. Epicorazine B was present in the extracts of only the two most active strains.