Microanalytical studies of metal localization in biological tissues by environmental SEM
作者:
Louise M. Egerton‐Warburton,
Brendon J. Griffin,
John Kuo,
期刊:
Microscopy Research and Technique
(WILEY Available online 1993)
卷期:
Volume 25,
issue 5‐6
页码: 406-411
ISSN:1059-910X
年代: 1993
DOI:10.1002/jemt.1070250509
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Bulk frozen hydrated samples;Eucalypts;Aluminium;Manganese
数据来源: WILEY
摘要:
AbstractThe presence and distribution of Al and Mn in floral and seed tissues of eucalypts from Al‐contaminated soils was analyzed using energy‐dispersive X‐ray microanalysis (EDS) in an environmental scanning electron microscope (ESEM). EDS by ESEM determined the distribution of elements between tissue types was suitable for intact samples or those with lower available moisture or intact specimens. The analytical technique was not appropriate for highly vascular samples. Other factors influencing the detection of elements by ESEM‐EDS were electron scattering and the relative concentration and localization of elements within the tissues. EDS‐detectable levels were significantly correlated with tissue concentrations determined by atomic absorption spectrophotometry for Mn but not for Al. © 1993 Wiley
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