This study reports the relationship between N levels (22, 56 and 112 kg/ha) and its effect on yield and protein quality for five cultivars of soft winter wheat (Triticum aestivumL. em. Thell). The objective was to determine the relationship of amino acid content and total protein at various levels of N. The 56 and 112 kg N/ha rates resulted in higher wheat yields than the 22 kg N rate over the 3 yr. mean. A cultivar x N level interaction for yield occurred when certain cultivars did not respond to higher N rates in 1972. Total seed protein increased with each increase in If. Increasing N levels generally resulted in a higher amlno acid content of the seed. Cultivar effects were found for 6 of the 17 amino acids (threonine, serine, glutamic acid, proline, glycine and lysine) indicating that cultivars differed in total amino acid content. Cultivars Arthur and Holley gave the greatest response (percentage amino acid) to N application. Coker 68–8 was intermediate in response while Coker 68–15 and Blueboy showed small inconsistent responses to H application.