Rapid Quantitation of Verapamil in Plasma by High-Performance Liquid Chromatography
作者:
E.M. Niazy,
H.W. Jun,
期刊:
Analytical Letters
(Taylor Available online 1985)
卷期:
Volume 18,
issue 9
页码: 1103-1111
ISSN:0003-2719
年代: 1985
DOI:10.1080/00032718508069103
出版商: Taylor & Francis Group
关键词: Verapamil;Analysis;LC;Rabbits;Plasma Levels
数据来源: Taylor
摘要:
A simple and sensitive liquid chromatographic assay procedure using a fluorescence detector for the quantitative determination of verapamil in plasma without extraction was developed. After precipitating the protein with acetonitrile, the resulting supernatant liquid was injected onto the column for analysis. Chromatographic separation was achieved on C18reversed phase column and the eluting solvent was the isocratic mixture of methanol, acetonitrile and pH 3.0 glycine buffer (1:4:5). With this mobile phase the drug and its internal standard were well separated from the interference of the plasma sample. The average recovery of verapamil from 3 replicate samples of different concentration (100–600 ng/mL) were 95.5 ± 5.68%. The minimum amount of verapamil detectable by this method was 40 ng/mL of sample. The elimination half-life (β-phase) of this drug in rabbits was found to be 3.7 hours.
点击下载:
PDF (157KB)
返 回