AbstractUsing noninfectious Sendai virus preparations after selective enzymatic digestion of either of the two viral envelope glycoproteins, it was possible to study the effect of different virion‐cell membrane interactions on virus‐specific cytotoxic T lymphocyte (CTL) inductionin vitro. Three different virus preparations having capacity for virus‐ cell fusion, for virus‐cell adsorption or lacking the ability to bind to cell membranes, were all active in the generation of virus‐specific primary and secondary cytotoxic T cells, when added to the culture. Investigations on the responder cell requirements during CTL induction revealed that activation by addition of virions lacking the capacity to bind to cells was sensitive to the depletion of adherent cells. When virions with fusion and binding capacity were presented on tumor stimulator cells, different requirements with respect to adherent cells were obtained in the primary and secondary CTL response to Sendai virus. The data indicate that different viral antigen‐cell membrane interactions govern the activation phase and effector phase of antigen‐ primed T cell populations, while sensitization of unprimed cells is dependent on the presence of adherent, perhaps antigen‐p