Defective Cytokine Expression but Adult‐Type T‐Cell Receptor, CD8, and p56lckModulation in CD3− or CD2‐Activated T Cells from Neonates
作者:
H. PIRENNE-ANSART,
F. PAILLARD,
D. GROOTE,
A. ELJAAFARI,
S. GAC,
P. BLOT,
P. FRANCHIMONT,
C. VAQUERO,
G. STERKERS,
期刊:
Pediatric Research
(OVID Available online 1995)
卷期:
Volume 37,
issue 1
页码: 64-69
ISSN:0031-3998
年代: 1995
出版商: OVID
数据来源: OVID
摘要:
Expression of IL-2, interferon-γ, and IL-3 mRNA and proteins was investigated in peripheral blood mononuclear cells from cord blood after activation with phytohemagglutinin, CD2, or CD3 MAb. The results showed that interferon-γ and IL-3 expression was decreased in cord peripheral blood mononuclear cells when compared with expression observed in adult peripheral blood mononuclear cells, irrespective of the stimulation used. In addition, in newborn cells a defect in IL-2 secretion and mRNA expression was observed in response to CD2 or CD3 MAb but not in response to phytohemagglutinin-mediated activation. We further analyzed the modulation of nonlymphokine genes under the same protocol of stimulations. The results indicate that in newborn cells, despite a reduced lymphokine expression observed after CD2 or CD3 MAb activation, the up-regulation of the T-cell receptor, CD8, and p56lckwas similar to that found in adult cells, as was also found after phytohemagglutinin activation of both types of cells. These data are in favor of a deficient T-cell responsiveness to CD2 or CD3 MAb in newborn cells. This impairment of the T-cell response appears to selectively affect lymphokine gene expression because the modulation of other genes also implicated in T cell activation is not altered.
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