Resolution Optimization in Ion-Interaction RP-HPLC.
作者:
M.C. Gennaro,
R. Aigotti,
S. Angelino,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 12
页码: 2271-2278
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708001738
出版商: Taylor & Francis Group
关键词: resolution;liquid chromatography;ion-interaction RP-HPLC
数据来源: Taylor
摘要:
Since in ion-interaction chromatography the dependence of the retention on the concentration of the ion-interaction reagent (IIR) follows different behaviours for the different analytes, the adjustment of IIR concentration can usefully be used to optimize resolution. As an example, resolution between nitrate and p-aminophenol was investigated as a function of IIR concentration and mobile phase flow-rate. The IIRs used are an aqueous solution of octylamine, brought to pH=6.4 with o-phosphoric acid.
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