Histological Preparation of the Undecalcified Rat Otic Bulla for Mesoscopic Observations
作者:
LatasterL M. A.,
van GeestA.,
van den BergeH.,
DrukkerJ.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 2
页码: 93-98
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110558
出版商: Taylor&Francis
关键词: undecalcified bone;rat otic bulla;hollow specimens;methylmethacrylate;3-D graphic reconstruction
数据来源: Taylor
摘要:
Otic bullas of the rat, obtained by excision and formalin fixed, are successfully embedded in methylmethacrylate by dehydration and subsequent infiltration with plastic under vacuum. Sections 10μm thick are obtained by cutting the trimmed and sandpapered acrylic blocks on an LKB multirange microtome. The sections are collected on adhesive tape and stained with a Trichrome stain (modified Weigert-van Gieson). Finally, the sections attached to the tape are mounted on microscope slides with glycerin-gelatin and sealed in the same medium. Serial sections are used for three-dimensional graphic reconstruction.
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