A study of the interaction of sodium dodecyl sulphate with the proteins of human heel stratum corneum
作者:
ANDREA CONRADS,
HELMUT ZAHN,
期刊:
International Journal of Cosmetic Science
(WILEY Available online 1987)
卷期:
Volume 9,
issue 1
页码: 29-46
ISSN:0142-5463
年代: 1987
DOI:10.1111/j.1467-2494.1987.tb00458.x
出版商: Blackwell Publishing Ltd
关键词: heel callus;keratins;protein characterization;surfactant uptake
数据来源: WILEY
摘要:
SynopsisThe aim of this research is to study thein vitrointeraction of the anionic surfactant sodium dodecyl sulphate with human heel callus with special regard to the callus proteins.The sorption of SDS by callus is analysed at different pH values, demonstrating the expected maximum binding of the anionic surfactant at low pH. In both surfactant and blank solutions, swelling of the callus pieces occurs but to different extents. The sorption of SDS by callus is accompanied by a loss of free amino acids and proteins. The protein composition of the callus residues after chemical treatment and of the corresponding treatment baths is examined by amino acid analysis and shows differences in the respective molar amino acid ratios. Results obtained with more specific techniques, such as gel electrophoresis and immunoblotting, demonstrate identical as well as different protein components in the treatment baths depending on the experimental conditions (pH, blank or SDS). Although effects due to the surfactant treatment are in principle more distinct than with blank experiments, those of the latter cannot be neglected.
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