Dopamine D1-Like Receptor Stimulation Inhibits Hypertrophy Induced by Platelet-Derived Growth Factor in Cultured Rat Renal Vascular Smooth Muscle Cells
作者:
Kenichi Yasunari,
Masakazu Kohno,
Hiroaki Kano,
Koji Yokokawa,
Mieko Minami,
Junichi Yoshikawa,
期刊:
Hypertension
(OVID Available online 1997)
卷期:
Volume 29,
issue 1
页码: 350-355
ISSN:0194-911X
年代: 1997
出版商: OVID
数据来源: OVID
摘要:
Vascular smooth muscle cell (VSMC) hypertrophy is believed to play some roles in atherosclerosis. To elucidate the role of vascular D1-like receptors in VSMC hypertrophy, the effects of dopamine and specific D1-like receptor agonists SKF 38393 and YM 435 on platelet-derived growth factor (PDGF) BB-mediated VSMC hypertrophy was studied. We observed that cells stimulated by PDGF-BB 5 ng/mL showed increased VSMC hypertrophy. These effects were prevented by coincubation with dopamine, SKF 38393, and YM 435 1-10 micro mol/L, and this prevention was reversed by Sch 23390 1 to 10 micro mol/L, a specific D1-like receptor antagonist. These actions are mimicked by forskolin 1 to 10 micro mol/L, a direct activator of adenylate cyclase and 8-bromo-cAMP 0.1 to 1 mmol/L, and are blocked by a specific protein kinase A (PKA) inhibitor N-[2-(P-bromocinnamylamino)ethyl]-5-isoquinoline-sulfonamide (H 89) but not blocked by its negative control. PDGF-BB (5 ng/mL)-mediated mitogen-activated protein kinase (MAPK) activity was significantly suppressed by coincubation with D1-like receptor agonists, which were reversed by PKA inhibitor H 89. These results suggest that vascular D1-like receptor agonists inhibit hypertrophy of VSMC, possibly through PKA activation and suppression of activated MAPK activity. (Hypertension. 1997;29[part 2]:350-355.)
返 回