Differentiation of Y79 Retinoblastoma Cells with Pigment Epithelial-Derived Factor and Interphotoreceptor Matrix Wash: Effects on Tumorigenicity
作者:
SeigelGail M.,
TombranJoyce,
BecerraS. Patricia,
ChaderGerald J.,
DiloretoDavid A.,
CerroConstancia Del,
LazarEliot S.,
CerroManuel Del,
期刊:
Growth Factors
(Taylor Available online 1994)
卷期:
Volume 10,
issue 4
页码: 289-297
ISSN:0897-7194
年代: 1994
DOI:10.3109/08977199409010995
出版商: Taylor&Francis
关键词: retinal transplantation;neurite outgrowth;retina;human tumors;tissue culture
数据来源: Taylor
摘要:
AbstractWe investigated thein vivodifferentiation potential of Y79 human retinoblastoma cells following pre-treatment with two novel neurotrophic agents: PEDF (human recombinant pigmented-epithelial derived factor) or IPM (interphotoreceptor matrix) wash. These agents were able to induce a significant degree of morphological differentiationin vitro. However, 48 days after subretinal transplantation of pre-treated cells, massive tumor formation was apparent. In contrast, Y79 cells pre-treated with retinoic acid/sodium butyrate, which attain a lesser degree of morphological differentiation, did not produce tumors over a 30 to 60 day-survival time (del Cerro et al.,Brain Research, 12–22, 1992). We conclude that for PEDF and IPM, the degree ofin vitrodifferentiation and the degree of mitotic arrest are independent features.
点击下载:
PDF (4029KB)
返 回