Disposable Sensing Elements for Fluorescent Immuno-Displacement Assays
作者:
Andrey L. Ghindilis,
Emmanuil Rabinovich,
Gabriel P. Lopez,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 8
页码: 1565-1573
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542916
出版商: Taylor & Francis Group
关键词: Immuno-displacement;Fluorescence;Monolayer;Immuno-assay;Enkephalin
数据来源: Taylor
摘要:
An immuno-displacement assay technique utilizing disposable sensing elements and based on fluorescence detection of rhodamine labeled immuno-complexes has been developed. Enkephalin has been used as a model analyte. The disposable sensing element is a strip coated with a thin gold layer by vacuum evaporation. The gold surface is modified by immobilized enkephalin. The enkephalin layer is treated with a rhodamine labeled immuno-complex against enkephalin to form a fluorescent layer. A presence of free enkephalin in the media causes a displacement of the labeled immuno-complex. This results in a decrease in surface fluorescence, which is proportional to the concentration of the free enkephalin (analyte). A detection range 1-100 μM for the enkephalin assay has been observed for a 10 min assay time.
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