Sequence and Cognitive Analyses of Two Virulence-Associated Markers of Bluetongue Virus Serotype 17
作者:
Kristen A. Bernard,
Barbara A. Israel,
William C. Wilson,
Kevin T. Schultz,
期刊:
Intervirology
(Karger Available online 1997)
卷期:
Volume 40,
issue 4
页码: 226-231
ISSN:0300-5526
年代: 1997
DOI:10.1159/000150551
出版商: S. Karger AG
关键词: Bluetongue virus;Sequence analyses;Virulence subject index;Virulence markers
数据来源: Karger
摘要:
Genome segments 2 and 3 were completely sequenced for one virulent and one avirulent bluetongue serotype 17 (BLU-17). These two segments were previously shown to exhibit virulence-associated markers. The marker on segment 2 was characterized as a change in the neutralization domain on its protein product, VP2. The nucleotide sequences for segments 2 were 94.5% identical, and their predicted proteins differed by 34 amino acids or 3.7%. Three clusters of variability were identified which may be involved with viral neutralization. These variable regions were compared to mutations for published monoclonal antibody-resistant variants of BLU. The marker on segment 3 was characterized as a mobility shift in polyacrylamide gel electrophoresis (PAGE). The nucleotide sequences were 95.0% identical, and their predicted proteins differed by four amino acids or 0.4%. These amino acid changes were relatively conserved; therefore, they are not likely responsible for virulence. The segment 3 sequences were compared to published sequences, and evidence was found to suggest that the virulent isolate had naturally reassorted between a BLU-17 and BLU-10 isolate.
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