Mutational Analysis of HBsAg Assembly
作者:
Reinhild Prange,
Constance M.T. Mangold,
Ralf Hilfrich,
Rolf E. Streeck,
期刊:
Intervirology
(Karger Available online 1995)
卷期:
Volume 38,
issue 1-2
页码: 16-23
ISSN:0300-5526
年代: 1995
DOI:10.1159/000150410
出版商: S. Karger AG
关键词: Hepatitis B virus;Surface antigen;Viral envelope;Site-directed mutagenesis;Membrane translocation;Cysteine mutants;Endoplasmic reticulum;Topogenic signal;Myristylation
数据来源: Karger
摘要:
The 20-nm noninfectious empty envelope particles carrying the hepatitis B surface antigen are secreted in large excess from hepatocytes during a hepatitis B infection. Similar particles produced in cell lines or yeast are an efficient vaccine against hepatitis B virus. We have analyzed the assembly of 20-nm particles using a mutagenesis approach. Specific mutations were introduced into the S gene and the preS region encoding the viral envelope proteins using recombinant DNA techniques. The mutant genes were expressed in cell lines to identify the amino acid residues that are critical for the assembly and the secretion of the 20-nm particles.
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