Isolation and identification of AKH/RPCH family peptides in blister beetles (Meloidae)
作者:
GERD GÄDE,
期刊:
Physiological Entomology
(WILEY Available online 1995)
卷期:
Volume 20,
issue 1
页码: 45-51
ISSN:0307-6962
年代: 1995
DOI:10.1111/j.1365-3032.1995.tb00799.x
出版商: Blackwell Publishing Ltd
关键词: AKH/RPCH‐peptide family;blister beetles;Decupotoma lunata;sequence elucidation
数据来源: WILEY
摘要:
Abstract.Two peptides were isolated from methanolic extracts of corpora cardiaca of the blister beetle,Decupotoma lunata, by a single‐step purification procedure, utilizing C‐18 reversed‐phase high‐performance liquid chromatography (RP‐HPLC) for separation, and the increase of haemolymph lipids inLocusta migratoriafor bioassay. The native peptides were analysed by matrix‐assisted laser‐desorption ionization mass spectrometry revealing main ions at m/z 1180 and 1009 respectively which were attributed to the [M + Na]+form of the respective peptides. After deblocking of the N‐terminal pyroglutamate residue of each peptide, the structures of the deblocked peptides were determined by pulsed‐liquid phase sequencing employing Edman chemistry. The sequences of the two peptides, (1) pGlu‐Leu‐Asn‐Phe‐Ser‐Pro‐Am‐Trp‐Gly‐AsnNH2and (2) pGlu‐Leu‐Asn‐Phe‐Ser‐Pro‐Asn‐TrpNH2, characterize them as deca‐ and octapeptide members of the AKH/RPCH family. Whereas the decapeptide is a novel member of this family and is given the acronym Del‐CC (Decupotoma lunatacorpus cardiacum peptide), the octapeptide has previously been found in tenebrionid beetles and has the acronym Tem‐HrTH. The corpora cardiaca of two other species of blister beetles (Cyaneolytta pectoralisandMylabris coeca) contain the same two peptides asD. lunata, as judged by RP‐HPLC and biological activity. Neither a corpus cardiacum extract ofDecupotoma lunatanor the synthetic peptides Del‐CC and Tem‐HrTH were activ
点击下载:
PDF
(563KB)
返 回