SummaryWe investigated methods for measurement of β2‐microglobulin, lysozyme, IgG and other globulin fragments such as Fc, F(ab')2,K‐and Δ‐chains in unconcentrated urine. A log‐log correlation was observed between the percent transferrin clearances and the molecular weights of β2‐microglobulin, lysozyme, transferrin and IgG in normal children by quantitative analysis. Glomerular damage was suggested in patients who excreted urinary protein in excess of 1 g/m2/ day. The selectivity patterns showed persistence of glomerular disorders in patients with membranoproliferative glomerulonephritis, crescentric glomerulonephritis, glomerulonephritis due to Henoch‐Schonlein purpura and lupus nephritis, in spite of decrease in urinary protein excretion after treatment.Scanty globulin fragments were found in urine. The ratio of dimer‐ and monomer‐light chains found varied depending on the pathogenesis.Electrophoretic figures seen by the Western blot method were able to differentiate urinary proteins using unconcentrated urine.A combination of the procedures in this report could be useful to evaluate the severity and to monitor the progr